Oral administration of sodium fluoride (40 mg/kg body weight) from day 6 to 19 of gestation caused, as comparedto control, significant reductions in body weight, feed consumption, absolute uterine weight and number of implantations. Significantly higher incidenceof skeletal (wavy ribs, 14th rib, <6 sternal centre, dumbell-shaped second and fifth sternebrae, incompleteossification of skull and thickening of tibia) and visceral (subcutaneous haemorrhage) abnormalities were also observed in NaF-treated dams than that of control. Oral administration of vitamin C (50 mg/kg bodyweight) and vitamin E (2 mg/0.2 ml olive oil/animal/day) from day 6 to 19 of gestation along with NaF significantly ameliorates NaF-induced reductions in body weight, feed consumption, absolute uterine weight (only with vitamin E treatment) and number of implantations. Ascompared with NaF-treated alone, the total percentage of skeletal and visceral abnormalities were significantly lowered in fluoride plus vitamin C-treated animals. Vitamin E was less effective.
These findings suggest that Vitamin ‘C’ significantly reduced the severity
and incidence of fluoride-induced embryo toxicity in rats.